Collection: Protein Expression & Purification

Both chelate nickel to capture polyhistidine tags; IDA holds the metal with three coordination sites and NTA with four. Takara's His60 Ni is a Ni-IDA resin with a 60 mg/ml binding capacity for one-step capture from bacterial, mammalian, or baculovirus lysates, while Cytiva's HisTrap HP columns come prepacked with Ni Sepharose HP. Imidazole gradients need re-optimizing whenever you switch resin chemistry.

Yes — gravity-flow columns, magnetic beads, and membrane-based spin formats all work at bench scale. Takara's Capturem kits capture his-tagged protein on a nickel-charged membrane in a standard centrifuge, going from clarified lysate or culture supernatant to purified protein in minutes, in miniprep, maxiprep, and 96-well formats.

The standard strategy is capture, intermediate purification, polish: an affinity step such as HisTrap does the heavy lifting, ion exchange removes closely related contaminants when needed, and size exclusion — Superdex, for example — clears aggregates and exchanges buffer. Sephadex desalting columns bridge steps when buffers must change.

When reproducibility, gradient control, or documentation start to matter — an ÄKTA go runs programmed methods with UV and conductivity monitoring in a footprint that fits a cold cabinet, which manual gravity columns cannot match. Labs purifying the same construct repeatedly usually recoup the bench time quickly.

Both are vapor-diffusion methods; sitting drop is easier to automate and gentler on fragile drops, while hanging drop simplifies crystal retrieval. Greiner's 96-well CrystalQuick plates run sitting-drop screens with three wells per reservoir — 288 conditions per plate — and the CrystalDrop lid lets one plate serve both geometries.

Danyel Biotech is the official Israeli distributor for the Cytiva, Takara Bio, and Greiner Bio-One protein-research lines — ÄKTA instruments, his-tag purification kits, and crystallization plates ship with local support and fast quotations.