pCold™ ProS2 DNA

Takara's pCold ProS2 DNA is a cold shock expression vector that includes Protein S derived from a Gram-negative myxobacteria Myxococcus xanthus as a solubilization tag. Protein S, (173 aa) is very stable soluble protein present in the spore surface coat of Myxococcus xanthus. Fusing a target gene encoding a protein of interest with the ProS2 Tag, found in pCold ProS2 DNA may induce the stability and solubility of the fusion protein.

Features

• High yield protein expression with optimized protein folding
• Enables expression of fusion proteins with soluble tag to optimize soluble protein production

Applications

Protein expression utilizing the promoter of cold-shock gene (cspA)

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pCold ProS2 DNA Vector sequence file in GenBank format