pT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES His-N DNA is an expression vector including a his-tag sequence. Using pT7-IRES His-N DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as an N-terminal fusion with a his tag. A Factor Xa cleavage site is included so that the his tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.
Features
They allow quick analyses of protein functions or features.
They are amenable to high-throughput studies.
Proteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.
Applications
Expression of toxic proteins that are lethal for in vivo expression systems Quick analysis of protein functionality Rapid analysis of protein mutations or truncations Expression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli High-throughp
