Collection: Oligos & Primers

M13 forward and reverse primers anneal to fixed sites flanking the polylinker in M13mp and pUC-derived vectors, so one pair sequences almost any insert cloned into them. That is why they remain the standard for clone verification — no primer design per construct, and no synthesis wait, since they are stocked as catalog oligos.

A primer is a short single-stranded oligo that anneals to a template so a polymerase can extend from it. A linker is a short double-stranded oligo carrying a restriction site, ligated onto blunt ends to create a cloning handle. An adaptor is similar but arrives with a compatible overhang already formed, so it ligates directionally — the basis of directional cDNA cloning and library construction.

Phosphoramidite synthesis builds the oligo base by base on a solid particle, and the support's loading capacity determines how much product a given column delivers. Cytiva's Primer Support 5G is a cross-linked polystyrene support loaded at 350 µmol/g for DNA and 300 µmol/g for RNA, supporting oligos of around 70 bases and scale-up from 1 µmol to 750 mmol.

Not from these listings. This category holds catalog primers, linkers and cloning vectors, plus the consumables used to manufacture oligos — it is not a custom synthesis service. For custom primers, probes or modified oligos, contact Danyel Biotech directly and we will route the request to the right supplier and come back with a quote.