Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).
Features
Expression of difficult proteins that can not be expressed with the T7 system
Increased solubility due to expression at reduced temperature
Increased purity due to repressed expression of host proteins
When used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased
Radioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein
Applications
High-efficiency protein expression using a cold-shock promoter
