xTractor Buffer is an optimized buffer system for extraction of proteins from fresh or frozen bacterial cells, mammalian cells, yeast cells, or baculovirus-infected cells. This buffer is useful in extracting proteins over a broad range of molecular weights, including high molecular weight proteins that cannot be extracted unless the membrane is fully disrupted. Lysozyme and DNase I are included to help disrupt membranes and to reduce solution viscosity, respectively. xTractor Buffer is compatible with all resins, allowing quick purification of his-tagged proteins. Sufficient buffer is provided for 10 extractions of 1 g cell pellets.
Features
Extract proteins from bacterial, yeast, mammalian, and baculovirus-infected cells
Fast, easy procedure—requires only a 10-min incubation
