Preparation and Specification
Appearance: Reddish brown amorphous powder, lyophilized
Activity: GradeⅡ 10U/mg-solid or more
Contaminants: Catalase ≤5%
Adenosine deaminase ≤1.0×10⁻³%
Uricase ≤1.0×10⁻³%
Phosphatase ≤1.0×10⁻³%
Purine-nucleoside phosphorylase ≤5.0×10⁻³%
Stabilizers: Sodium glutamate, BSA
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 160,000
Isoelectric point: 4.0±0.1
Michaelis constants: 4.5×10⁻⁵M (Xanthine), 7.6×10⁻⁵M (Hypoxanthine)
Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA
Optimum pH: 7.5-8.0
Optimum temperature: 65°C
pH Stability: pH 6.5-9.0 (25°C, 15hr)
Thermal stability: below 55°C (pH 8.0, 30min)
Applications
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase and uricase.
Research use only.
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