Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: 5.74U/mg
Contaminant: Catalase ≤1.0%
Stabilizers: Borate, EDTA, nonionic detergents
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 120,000
Structure: 4 subunits per enzyme molecule (Reactive SH groups are present in the enzyme molecule)
Isoelectric point: 5.4
Michaelis constant: 2.5×10⁻⁵M (Uric acid)
Inhibitors: Heavy metal ions, cyanide,various urate analogs
Optimum pH: 8.5
Optimum temperature: 40°C
pH Stability: pH 7.0-11.0 (25°C, 20hr)
Thermal stability: below 50°C (pH 8.5, 10min)
Applications
This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Research use only.
Documents
