Urea (carbamide, carbonyl diamide) is a predominant final metabolite of nitrogenous compounds in mammals, accounting for 80-90% of nitrogen excretion in human and animals. Formed exclusively in the liver, urea is mostly transported by the bloodstream to the kidneys, where it is excreted into the urine. It is an indicator of liver and kidney functions. Determination of urea concentration is an essential task in clinical laboratories and research.
The TriboTM Urea Colorimetric Assay provides a rapid, sensitive method to measure urea concentration in biological samples. The intensity of the color is directly proportional to the urea concentration in the sample.
Applications
Direct Assay: urea in serum, plasma, urine, milk, cell/tissue culture, animal products and wine.
Drug Discovery and screen: effects of drugs on urea metabolism.
Key Features
Sensitive and accurate: The Kit detects as low as 0.1 mg/dL in solution.
Resistant to ammonia interference, a common problem in urease-based assays.
Works reliable in complex matrices such as phenol red-containing media (e.g., cell culture media).
Simple and high-throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.
Kit Contents for 200 assays
Component
Unit Size
Urea Standard 500 mg/dL)
200 µL
Urea reagent Component A
22 mL
Urea reagent Component B
22 mL
Component C: Assay Buffer
30 mL
Storage Conditions
Store the kit at 4°C. For long-term storage, keep the standard at -20 °C. Shelf-life: 12 months.
