Ultra-Sensitive TMB Substrate is one bottle stabilized chromogenic substrate for horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one bottle format with long term stability and high sensitivity. It develops a deep blue color in the presence of HRP. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA, Western blotting, and immunohistochemistry procedure. The addition of sulfuric acid stop solution (TBS5030) changes the color to yellow, enabling accurate measurement of the intensity at 450 nm using a spectrophotometer or plate reader. This formulation is used in most of the Tribioscience ELISA Kits where it provides consistent performance for the ELISA technique. It is a ready-to-use formulation with NO DMF or DMSO.
Storage Conditions
It is highly stable for a minimum of 36 months when stored at 4℃.
Research use only.
Documents
References
Chen, J., Tran, T., Wong, A., Wang, L., Annaluru, P., Sreekanth, V., Murthy, S., Munjeti, L., Park, T., Bhat, U., Leong, G., Li, Y., Chen, S., Kong, N., Raval, R., Xie, Y., Somani, S., Bhambhani, A. M., Zhu, Z., … Chen, Z. (2025). Direct Expression of CPT1a Enables a High Throughput Platform for the Discovery of CPT1a Modulators. Applied Biosciences, 4(2), 25. https://doi.org/10.3390/applbiosci4020025
Research use only.
