Tribo™ Tryptase Activity Assay

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules, comprising up to 20% of the total protein of mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.

The Tribio^TM Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluation of tryptase activity in cell lysates, supernatants or for inhibitor screening.

Applications

• Cell culture supernatants, cell lysates, or other tryptase-containing supplies.
• Testing of purified tryptase enzyme, in vitro inhibitor screening.
• Cell degranulation

Key Features

• Sensitive and Accurate: The kit detects as low as 10μM in solution.
• Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

Kit Contents (100 assay)

Note: Positive Control is not included in the kit. We recommend that our tryptase recombinant α/β-1 protein enzyme (catalog: TBP0099). This enzyme was validated with this kit.

Storage Conditions:

Store the kit at -20℃, protect from light.
Shelf life: 12 months.

Standard Preparation (Fig.1)

Fig. 2: pNA Standard Curve

Fig. 3: Tryptase Positive Control Curve

Fig. 4: Effects of pH Value on Tryptase Activity 

Alternative names: Mast Cell Tryptase, Tryptase enzyme