The kit provides a straightforward, metal affinity-based procedure for isolating microgram quantities of phosphorylated proteins from mammalian cells and tissues. It supplies a complete set of buffers along with phospho-specific magnetic beads for enrichment of all types of phosphoproteins, both cytosolic and membrane-bound, that contain a phosphorylated amino acid side chain-including serine, threonine, or tyrosine.
Features
A simple, metal affinity-based procedure for microscale phosphoprotein purification
Quick and easy enrichment from any cell or tissue sample
Eluted samples ideal for small volume applications
Applications
Isolation and enrichment of all types of phosphoproteins.