T4 RNA Ligase catalyzes the formation of phosphodiester bonds between singlestranded nucleic acids with 5'-phosphate and 3'-hydroxyl termini. The enzyme requires ATP for activity. The rate of activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation and very low for DNA-DNA ligation. T4 RNA Ligase is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Cat. # 2050B contains 5 of Cat. # 2050A. Please refer to Cat. # 2050A for complete product documentation and resources.
Features
Ligation of single-stranded RNA or DNA
3'-end labeling of single stranded RNA or DNA
Applications
Ligation of single-stranded RNA or DNA 3'-end labeling of single stranded RNA or DNA
Unit definition
One unit is defined as the amount of enzyme that converts 1 pmol of [5'-32P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA
