T4 DNA Polymerase

T4 DNA Polymerase catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'→3' direction. It possesses a strong 3'→ 5' exonuclease (proofreading) activity, but does not exhibit 5'→3' exonuclease activity. T4 DNA Polymerase is supplied in 200 mM potassium phosphate (pH 6.5), 1 mM DTT and 50% glycerol.

Features

• Generation of blunt double-stranded DNA from DNA containing 5'/3' overhangs
• 5'-end or 3'-end labeling of double-stranded DNA
• In vitro mutagenesis

Applications

Generation of blunt double-stranded DNA from DNA containing 5'/3' overhangs
5'-end or 3'-end labeling of double-stranded DNA
In vitro mutagenesis

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)