T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of ATP. T4 DNA Ligase is supplied in 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Cat. # 2011B contains 5 of Cat. # 2011A. Please refer to Cat. # 2011A for complete product documentation and resources.
Features
Blunt and cohesive end DNA ligation
Blunt end linker addition
Single-strand DNA, RNA and DNA/RNA repair
DNA fragment insertion into vectors
Applications
Blunt and cohesive end DNA ligation Blunt end linker addition Single-strand DNA, RNA and DNA/RNA repair DNA fragment insertion into vectors
Unit definition
One ligation unit is defined as the amount of enzyme that ligates >90% of 6 µg of Hind III-restricted lambda DNA in a 20 µl reaction volume in 30 minutes at 16°C. One unit of the enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in
