T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of ATP. T4 DNA Ligase is supplied in 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol.

Features

• Blunt and cohesive end DNA ligation
• Blunt end linker addition
• Single-strand DNA, RNA and DNA/RNA repair
• DNA fragment insertion into vectors

Applications

Blunt and cohesive end DNA ligation
Blunt end linker addition
Single-strand DNA, RNA and DNA/RNA repair
DNA fragment insertion into vectors

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)