Takara's Synthetic siRNA Quantitation Core Kit is designed for siRNA quantitation when used in combination with real time PCR analysis to assay residual siRNA in total RNA samples extracted from cells, tissues, blood and other specimens. There are two kits available, the Synthetic siRNA Quantitation Core Kit (Cat. #6142) and the Synthetic siRNA Quantitation Kit (with TB Green Premix Ex Taq II Tli) (Cat. #RR861A containing Cat. #6142 and Cat. #RR820A).
Features
This kit is designed for siRNA quantitation of molecules containing 3' d(TT) overhangs.
For synthetic siRNA with 3ʼoverhangs other than d(TT), prepare a specific oligo dT primer containing the modified sequence.
This product does not work with synthetic siRNAs that carry no 3’ overhangs or with RNA 3’ overhangs.
Applications
siRNA quantitation in total RNA with intercalating green dye Real Time PCR (qPCR) Low-copy-number siRNA quantitation
