The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–10 ng inputs of purified total RNA. This kit incorporates Takara Bio’s proprietary SMART (Switching Mechanism at the 5’ end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. This method was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. The new library design featured in the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian improves sequencing performance compared to the original SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian, particularly for NextSeq® and MiniSeq™ instruments carrying the two-channel SBS technology. This kit includes the Indexing Primer Set HT for Illumina v2; for your convenience, we also offer the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
Features
For 10–100 ng of total RNA, we recommend SMART-Seq Total RNA Mid Input
For 100 ng–1 µg of total RNA, we recommend SMART-Seq Total RNA High Input (RiboGone Mammalian)
Applications
Robust NGS library construction that retains strand information Use for RNA-seq on all Illumina platforms Captures coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
