What are the Sera-Mag™ Carboxylate-Modified Magnetic Beads & SpeedBeads? Sera-Mag™ Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH 2 ).
- Wide application range: Carboxylic groups on the surface form covalent amide bonds with proteins, peptides or amine-modified ligands for use in many downstream applications including protein purification, DNA sample preparation and clean-up, protenomicsand immunoassays.
- Convenient one-step or two-step coupling: Carbodiimide chemistry allows rapid formation of stable bonds to minimize ligand leaching.
- Fast magnetic separation: Available in original Sera-Mag™ format or Sera-Mag™ SpeedBeads version for faster magnetic response and shorter assay times in clinical diagnostic tests.
- High performance: Excellent sensitivity and low nonspecific binding for greater accuracy.
Sera-Mag™ SpeedBead carboxylate-modified magnetic particles now available in 1 µm and 3 µm nominal diameters.
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Find out which magnetic bead chemistry meets your application requirements in Magbeads 101: A guide to choosing and using magnetic beads and explore ways to optimize your magnetic bead workflow in Scientists guide to magnetic beads
What is the difference between E3 and E7 bead?
E3 and E7 refer to the different manufacturing process for the beads. E3 and E7 beads behave similarly and we continue to provide both bead types to our customers for test and validation in their chosen application.
How to covalently couple proteins to carboxylate-modified magnetic beads
The convenient “one-step” protocol for covalent coupling of proteins to Sera-Mag™ carboxylate-modified particles or Sera-Mag™ SpeedBeads carboxylate-modified particles involves the following steps
- Prepare a reagent mixture containing MES buffer, protein solution, and magnetic particles, and mix for 15 minutes at room temperature on a mixing wheel or other device.
- Add freshly prepared EDAC solution at the pre-determined optimal concentration, and mix for one hour at room temperature on a mixing wheel or other device.
- Pellet particles by centrifugation (10 to 30 minutes in a standard microcentrifuge) and decant the supernatant.
- Perform two washes with MES buffer or a high pH buffer of your choice, pelleting particles by centrifugation and using ultrasonication to resuspend pellets between washes.
- Resuspend final pellet to desired % solids with a buffer such as MES that does not contain blocking proteins.
- Perform a BCA protein assay to determine the amount of protein bound on the particles.
Have you considered your magnetic separation rack?
Cytiva’s Magnetic Separation Rack 15 mL is a 3D printed, magnetized tube rack with a strong, removable magnetic force and easy pipette access. It facilitates high capture, retention, and release of magnetic beads from various liquid sample media, allowing high-throughput, parallel processing of up to six 15 mL samples.
Find out more about magnetic separation racks
Check out Cytiva Diagnostic Services, helping you build and accelerate the development of smarter molecular and immunodiagnostic assays.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
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