S1 Nuclease specifically degrades single-stranded nucleic acids, including single-stranded regions of duplex DNA or RNA. S1 Nuclease is supplied in 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol. Cat. # 2410B contains 5 of Cat. # 2410A. Please refer to Cat. # 2410A for complete product documentation and resources.
Features
Nuclease protection assays
Double-stranded DNA end blunting
RNA transcript mapping
cDNA hairpin removal
Applications
Nuclease protection assays Double-stranded DNA end blunting RNA transcript mapping cDNA hairpin removal
Unit definition
One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into an acid-soluble product in 1 minute at 37°C and pH 4.6