rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.
- For monoclonal and polyclonal antibody purification at both laboratory and process scale.
- Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
- Free from animal-derived components.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Successful use in published research
Cytiva's protein A resins are used in a variety of applications, including:
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Convenient prepacked columns – research scale
rProtein A Sepharose Fast Flow is also available in convenient prepacked formats:
- rProtein A GraviTrap, for use with gravity flow
- HiTrap rProtein A FF, for use with a syringe, pump, or chromatography system
Convenient prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.
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