Ribonuclease H is an endoribonuclease that specifically degrades the RNA strand in an RNA-DNA hybrid. Ribonuclease H is supplied in 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol. Cat. # 2150B contains 5 of Cat. # 2150A. Please refer to Cat. # 2150A for complete product documentation and resources.
Features
Removal of RNA template following second-strand cDNA synthesis
Removal of mRNA poly(A) sequence
Reverse transcription
Applications
Removal of RNA template following second-strand cDNA synthesis Removal of mRNA poly(A) sequence Reverse transcription
Unit definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate
