RetroNectin® Precoated Dish

Pack of ten 35 mm dishes that are pre-coated with RetronNectin reagent. RetroNectin reagent is a 63 kD fragment of recombinant human fibronectin fragment (also referred to as rFN-CH-296) that enhances the efficiency of lentiviral- and retroviral-mediated gene transduction. This is particularly important for hematopoietic cells and other hard-to-transfect cell types. Enhanced transduction is thought to result from co-localization of virus particles and target cells. This is accomplished by direct binding of viral particles to sequences in the heparin-binding domain and interaction of target-cell integrins with two other domains in rFN-CH-296.

Features

• Enhanced gene transfer efficiency for retroviral or lentiviral transduction of suspension cells, hematopoietic stem cells, and other hard-to-infect cell types
• Less toxicity than other commonly used transduction enhancers, including polybrene and protamine
• Fully recombinant peptide
• The efficiency of T cell (CD8+) expansion can be significantly increased in the presence of RetroNectin reagent
• Takara Bio Inc., has submitted a Drug Master File (DMF application number 18898) to the Food and Drug Administration for RetroNectin GMP grade.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)