Retro-X™ qRT-PCR Titration Kit

This Retro-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of MMLV-based retroviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

Features

• Measure retrovirus titers using TB Green-based, real-time PCR
• Retrovirus copy numbers are determined by comparing the sample's Ct to a standard curve derived from a calibrated control template
• Confirm the success of your retroviral packaging reaction to avoid wasting time with your experiments
• Perform consistent experiments, control copy number, and hence expression levels
• Ensure sufficient MOI so that 100% of your cells are transduced

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Retro-X qRT-PCR Titration Kit Components List