Preparation and Specification
Appearance: Light brown amorphous powder, lyophilized
Activity: GradeⅢ 3.0U/mg-solid or more
Contaminant: NADPH oxidase ≤1.0×10⁻¹%
Stabilizers: Sugars
Properties
Stability: Stable at -20°C
Molecular weight: approx. 600,000 (by gel filtration)
Michaelis constant: 1.85×10⁻⁵M (Protocatechuate)
Structure: Protein with nonheme iron
Inhibitors: Ag⁺, Hg⁺⁺
Optimum pH: 9.0
Optimum temperature: 60-65°C
pH Stability: pH 6.0-9.5 (25°C, 72hr)
Thermal stability: below 55°C (pH 7.5, 1hr)
Applications
This enzyme is useful for enzymatic determination of choline esterase when coupled with p-hydroxybenzoate hydroxylase.
Research use only.
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