Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range. The DNA-free nature of recombinant Proteinase K made it well-suited for isolating PCR and RT-PCR templates.
Main Features
High purity: >99.5% by reducing SDS-PAGE.
RNase and DNase: Free
DNA: Free Specific activity: ≥ 30 units/mg of protein. Wider pH range: The effective pH range of Proteinase K is pH 4.0~12.5.
Application
DNA, RNA, and phage purification.
Protein digestion.
Chromosomal DNA preparation for in situ hybridization, fingerprinting, colony or plaque hybridization, and pulse-field electrophoresis.
