PrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5'-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps. Because this product is highly resistant to various PCR inhibitors in blood and soil, it can be used to directly detect pathogens, including viruses and bacteria, in various biological specimens and to directly analyze gene expression in cells.
Features
One-step RT-qPCR premix for ease of use
Strong thermostability
High tolerance for PCR inhibitors (e.g., heparin from blood and humic acid from soil)
Compatible with direct addition of crude extracted RNA or samples to reaction mix
Suitable for all probe-based assays
Applications
Gene expression Pathogen detection (viruses and bacteria) Direct RT-qPCR with no intervening purification steps
