pluriSpin Human B-Cell Enrichment

pluriSpin human B-cell enrichment, for negative selection of cells, has been developed to isolate a clean, vital and untouched B-cell population from whole blood, buffy coat, cord blood, bone marrow or similar sample materials. The product labels the monocytes, T-cells, NK-cells and granulocytes, the B-cell fraction remain untouched.

Subsequent PBMC spin density gradient centrifugation is used to remove all labeled cells from the sample together with the red blood cells (RBCs) and granulocytes. The untouched and unlabeled B-cell fraction remains enriched in the interphase between the plasma and the density medium. We recommend PBMC Spin Medium as the density medium for DGM centrifugation. Alternatively, Ficoll, Lymphoprep or other comparable density media can be used without changing the protocol.

Features

• Negative selection – Unwanted cells are labeled with specific antibodies and then removed, while the desired cells remain unlabeled in the suspension and retain their natural properties.
• High purity and cell viability – The isolated cells remain functional and can be used directly for subsequent experiments such as flow cytometry (FACS), cell culture or molecular biological analyses.
• No magnetic beads or columns required – Unlike many other cell separation methods, pluriSpin does not require magnetic beads or columns, which simplifies the process and preserves cell integrity.
• Compatible – Purification by density centrifugation can be carried out using PBMC Spin Medium or comparable density media from other manufacturers, such as Lymphopreo, Ficoll or Pancoll.

Specifications

Ordering Information