pluriBead Mouse Ly6G Cell Separation

Ly6G pluriBeads are suitable for rapid, positive cell separation of Ly6G+ cells from murine sample material. The target-specific non-magnetic pluriBeads can be used without sample preparation for cell isolation, directly in blood (whole blood), buffy coat or cell cultures. For mixing the Ly6G specific pluriBeads with the sample material we recommend our universal mixing device the pluriPlix or a horizontal mixer.

During the incubation period, the Ly6G+ cells from the sample bind to the specific surface of the pluriBeads. The pluriBeads, with the bound cells, are then separated from all unbound cells using a pluriStrainer. In the final step, the target cells are gently detached from the pluriBeads using our detachment buffer.

The enriched, particle-free Ly6G+ cells are immediately available for further downstream applications such as cell culture experiments or FACS analyses. For the isolation of cell-specific RNA, DNA or proteins, the bound cells can be lysed directly on the pluriStrainer without the detachment step.

Bead Size

pluriBeads are available as S-pluriBead and M-pluriBead; S and M define the size of the microbeads.

• S-pluriBead – 32 µm in diameter, for a small number of target cells (< 1 × 10^6 cells / ml sample volume)
• M-pluriBead – 65 µm in diameter, for a high number of target cells (> 1 × 10^6 cells / ml sample volume)

Features

• No sample preparation – Use 200 µl - 45 ml whole blood sample material without prior density gradient centrifugation or red blood cell lysis.
• Fast, gentle cell isolation – Isolate cells in just 5 minutes (depending on the cell type and the amount of target cells available).
• Simultaneous cellisolation – Combine S- and M-pluriBeads and isolate two different cell types from one sample at the same time.
• Sequential cell isolation – Unbound cells in a sample are not lost, but can be concentrated and reused for isolation.

Specifications

Ordering Information