pluriBead Mouse CD45 Cell Separation

CD45 pluriBeads are suitable for rapid, positive cell separation of CD45+ cells from murine sample material. The target-specific non-magnetic pluriBeads can be used without sample preparation for cell isolation, directly in blood (whole blood), buffy coat or cell cultures. For mixing the CD45-specific pluriBeads with the sample material, we recommend our universal mixing device the pluriPlix or a horizontal mixer.

During the incubation period, the CD45+ cells from the sample bind to the specific surface of the pluriBeads. The pluriBeads, with the bound cells, are then separated from all unbound cells using a pluriStrainer. In the final step, the target cells are gently detached from the pluriBeads using our detachment buffer.

The enriched, particle-free CD45+ cells are immediately available for further downstream applications such as cell culture experiments or FACS analyses. For the isolation of cell-specific RNA, DNA or proteins, the bound cells can be lysed directly on the pluriStrainer without the detachment step.

Bead Size

pluriBeads are available as S-pluriBead and M-pluriBead; S and M define the size of the microbeads.

• S-pluriBead – 32 µm in diameter, for a small number of target cells (< 1 × 10^6 cells / ml sample volume)
• M-pluriBead – 65 µm in diameter, for a high number of target cells (> 1 × 10^6 cells / ml sample volume)

Features

• No sample preparation – Use 200 µl - 45 ml whole blood sample material without prior density gradient centrifugation or red blood cell lysis.
• Fast, gentle cell isolation – Isolate cells in just 5 minutes (depending on the cell type and the amount of target cells available).
• Simultaneous cellisolation – Combine S- and M-pluriBeads and isolate two different cell types from one sample at the same time.
• Sequential cell isolation – Unbound cells in a sample are not lost, but can be concentrated and reused for isolation.

Specifications

Ordering Information