PLT Spin Medium, Density Gradient Medium

PLT Spin density gradient medium is a ready-to-use, sterile solution for the enrichment of platelets (PLT) from fresh human whole blood. In combination with other density separation media such as PBMC, Leuko or Monocyte Spin, contamination with platelets can be reduced. The enriched platelets can be used for a variety of experiments and applications, especially in the fields of regenerative medicine, cell biology, hemostaseology and immunology.

Features

• Enrich – PLT Spin can be used to obtain platelet concentrates from small sample volumes.
• Ready to use – pluriSpin separation media can be used directly for cell separation.
• Deplete – PLT Spin can be used to remove unwanted platelets from samples, such as enriched PBMC or leukocytes.
• Combinable – pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.

Separation Scheme

Distribution of blood cell populations as a function of density
Blood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.

Target cell population with PLT Spin density centrifugation
The density of the separation media defines the cut-off for the cells. PLT Spin Medium has a cut-off of 1.060 g/ml. All cells with a lower density, such as thrombocytes (1), are enriched with PLT Spin Medium. All cells with a higher density, such as monocytes (2), lymphocytes (3), basophil granulocytes (4), neutrophil granulocytes (5), eosinophil granulocytes (6) and erythrocytes (7) pass through the PLT Spin Medium and are depleted. At first, the sample material is carefully layered onto the PLT Spin medium. Mixing of the two phases must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The layer of platelets is then transferred to a new tube and the cells are washed.

Before centrifugation
Preparation of leukocytes with Leuko Spin Medium. The sample material (1) is carefully placed on the Leuko Spin Medium (2).

After centrifugation
Layers after centrifugation. After centrifugation, the platelets form a white layer (2) on the PLT Spin Medium (3). Cells with a higher density, such as erythrocytes, granulocytes, lymphocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).

Specifications

Ordering Information