PBMC Spin Medium, Density Gradient Medium

PBMC Spin density gradient medium is a sterile, ready-to-use solution for the isolation of mononuclear cells (lymphocytes and monocytes) from human peripheral whole blood or buffy coat. In combination with Leuko Spin Medium, the mononuclear cells and granulocytes of a sample can be isolated simultaneously with high yield and purity using a double gradient. PBMC Spin can be used as an alternative to Ficoll-Paque, Pancoll or Lymphoprep without changing existing protocols. The enriched mononuclear cells can be used for a variety of downstream applications such as pluriBead, EasySep, MACS or stem cell research.

Features

• Ready to use – The pluriSpin separation media are ready to use and can be used directly for cell separation.
• Replace – Alternative products, such as Ficoll-Paque, Pancoll or Lymphoprep, can be substituted with PBMC Spin for the application of PBMC.
• Combinable – pluriSelect separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.
• Compatible – The enriched cells can be used for downstream applications such as MACS, SepMate, RosetteSep, MojoSort.

Separation Scheme

Distribution of blood cell populations as a function of density
Blood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.

Target cell population with PBMC Spin density centrifugation
The density of the separation media defines the cut-off for the cells. PBMC Spin Medium has a cut-off of 1.077 g/ml. All cells with a lower density, such as thrombocytes (1), monocytes (2) and lymphocytes (3) are enriched with PBMC Spin Medium. All cells with a higher density, such as basophil granulocytes (4), neutrophil granulocytes (5), eosinophil granulocytes (6) and erythrocytes (7) pass through the PBMC Spin Medium and are depleted. First, the sample material is carefully layered onto the PBMC spin medium. Mixing of the two phases must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The layer of mononuclear cells is then transferred to a new tube and the cells are washed.

BEFORE
Preparation of PBMC with PBMC Spin Medium. The sample material (1) is carefully placed on the PBMC spin medium (2).

AFTER
Layers after centrifugation. The PBMC (2) are located as a white layer on the PBMC spin medium (3). Cells with a higher density, such as erythrocytes, granulocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).

Specifications

Ordering Information