PBMC 24+ Spin Medium, Density Gradient Medium

PBMC 24+ Spin Density Gradient Medium is a sterile, ready-to-use solution developed for the isolation of cells from older sample material. If the samples are already older than 6 hours, the yields and purities with conventional separation media for the enrichment of PBMC are considerably poorer. In addition, the cells are sometimes unsuitable for downstream applications. With PBMC 24+ Spin Medium, the time window between sample collection and cell enrichment is extended.

Features

• Access to older sample material – PBMC 24+ Spin Medium enables the enrichment of cells from sample material that is already older than 12 hours.
• Ready to use – pluriSelect separation media are ready to use and can be used directly for cell separation.
• Compatible – The enriched cells can be used for downstream applications such as MACS, EasySep, RosetteSep or MojoSort.
• Combinable – PBMC 24+ Spin Medium can be combined with Leuko 24+ Spin Medium to form a double gradient for the simultaneous enrichment of two cell populations.

Separation Scheme

First, the sample material is carefully layered onto the PBMC 24+ spin medium. After the density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The layer of mononuclear cells is then transferred to a new tube and the enriched cells are washed.

BEFORE
Preparation of PBMC with PBMC 24+ Spin Medium. The sample material (1) is carefully placed on the PBMC 24+ Spin Medium (2).

AFTER
Layers after centrifugation. The PBMC (2) are located as a white layer on the PBMC 24+ spin medium (3). Cells with a higher density, such as erythrocytes, granulocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).

Specifications

Ordering Information