OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides (Figure. 1). OmniCleave Endonuclease has the same substrate specificity and yields the same products as Benzonase®, an enzyme derived from Serratia marcescens.
Applications
Removal of nucleic acids from cell lysates (reduction of viscosity) for improved handling and yield of protein preparations. Removal of trace contamination by nucleic acids in protein preparations. Removal of host DNA from phage preparations. Improved electrophoretic and chromatographic separation of proteins isolated from whole-cell lysates.
Figure 1. Removal of nucleic acids from cell lysates using OmniCleave™ Endonuclease. Cell lysates were prepared with or without OmniCleave Endonuclease from E. coli cells expressing human lactate dehydrogenase B (LDH) and E. coli alkaline phosphatase (AP). Two microliters of each of the cell lysates were separated by electrophoresis on a 1% agarose gel and the nucleic acids detected by ethidium bromide staining. Lane M, 1-kb ladder.
read more
Key features
Non-specific: Digests all types of DNA and RNA.
Flexible: Active within a broad range of conditions.
Customisable: Contact us for bulk or high concentration enzyme.
