T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.
Key features
Reliable transcription.
Specifications
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37 °C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
Unit Concentration 50,000 U/mL Purity (SDS-PAGE) >99% SS Exonuclease 500 U <0.1% released DS Exonuclease 500 U <0.1% released Endonuclease 500 U <10% converted RNase Contamination 500 U = none detected E. coli 16S rDNA Contamination 500 U <10 copies Storage -20 °C read more
Product Details
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.
