NextSeq 2000 P3 Reagents (300 Cycles)
Vendor: Illumina
SKU: 20040561
Low stock
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (300 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
NextSeq 1000/2000 reagents leverage XLEAP-SBS chemistry to enable a wide range of applications. These reagents offer:
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Sequencing output of 10–540 Gb per run, providing adjustable output based on project needs
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Multiple flow cell configurations that offer flexibility to support new and emerging applications
Sustainability and simplicity in one
Easy-to-use reagent cartridges deliver a streamlined workflow that reduces the chance for errors, lowers operating costs, improves recyclability, and minimizes waste. The fully-integrated cartridges include reagents, fluidics, and the waste holder, streamlining library loading and instrument use.
Simply prepare the reagent cartridge, insert the flow cell, load the library into the cartridge, and insert into the instrument.
Specifications
| Instruments | NextSeq 2000 System, NextSeq 1000 System |
|---|---|
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis, Single-read sequencing |
| Technology | Sequencing |
Required products
Use XLEAP-SBS P1 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 60 Gb. Maximum single-end reads per run: 100M.
Use XLEAP-SBS P2 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 240 Gb. Maximum single-end reads per run: 400M.
Use XLEAP-SBS P3 reagents on the NextSeq 2000 System only. Maximum output: 360 Gb. Maximum single-end reads per run: 1.2B.
Use XLEAP-SBS P4 reagents on the NextSeq 2000 System only. Maximum output: 540 Gb. Maximum single-end reads per run: 1.8B.
The NextSeq 1000/2000 Index Primer Kit, Read Primer Kit, and Read and Index Primers are optional but required when both custom sequencing primers and Illumina primers are used in a run.*
PhiX Control v3 is recommended for all runs, but is optional for well-balanced library types.
*For Illumina DNA PCR-Free Prep, the XLEAP-SBS cartridge already contains the primers, whereas the standard SBS cartridge does not.
Documents
- NextSeq 1000 and NextSeq 2000 Systems FAQ
- NextSeq 1000 and NextSeq 2000 Systems standard SBS reagent kit specifications
- XLEAP-SBS chemistry on the NextSeq 1000 and NextSeq 2000 Systems infographic
- Illumina single-cell RNA sequencing flyer
- Illumina oncology workflows flyer
- 16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems
- A shotgun metagenomics NGS workflow for assessing microbial populations in complex samples
- Brazilian Portuguese
Support Documentation
Specifications
- Instruments NextSeq 1000 SystemNextSeq 2000 System
- Nucleic acid type DNA, RNA
- Reagent type Cluster generation, Paired-end sequencing, Sequencing by synthesis, Single-read sequencing
- Sub category Sequencing reagents & flow cells
- Technology Sequencing
Components
- 20047304 — NextSeq 1000/2000 Resuspension Buffer
- 20027800 — NextSeq 2000 P3 Flow Cell
- 20038732 — NextSeq 2000 P3 RGT Cart 300 Cyc
- 20050639 — NEXTSEQ 1000/2000 ResuspensionBufferv1.5
links and documants
- Datasheet url Click here
- Source url Click here
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NextSeq 2000 P3 Reagents (300 Cycles)
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