N-Acetylneuraminic acid aldolase, Enzyme Activity

Preparation and Specification

Appearance: Yellowish amorphous powder, lyophilized

Activity: 10U/mg

Contaminants: Catalase ≤1.0%

NADH oxidase ≤1.0×10⁻³%

Stabilizers: Mannitol, EDTA

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 98,000

Isoelectric point: 4.6±0.1

Michaelis constant: 2.5×10⁻³M (N-Acetylneuraminic acid)

Structure: 3 subunits (approx. 35,000) per enzyme molecule

Inhibitors: p-Chloromercuribenzoate, SDS, Hg⁺⁺, Ag⁺

Optimum pH: 7.5-8.0

Optimum temperature: 70°C

pH Stability: pH 6.0−9.0 (10°C, 25hr)

Thermal stability: below 65°C (pH 7.5, 30min)

Applications

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)