MTT assay is one of the most versatile and popular assays. It is based on the cleavage of the yellow tetrazolium salt MTT to purple formazan crystal by metabolic active cells. The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The formazan color intensity is directly proportional to the number of living cells in the culture.
The MTT Cell Viability Assay Kit provides a convenient, sensitive, quantitative, and reliable assay method for the determination of the number of viable cells using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents, and screening other biologically active compounds. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness, and automation.
Applications:
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Cell proliferation: effects of cytokines, growth factor, and nutrients.
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Cytotoxicity and Apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
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Drug discovery: high-throughput screen for toxic and anticancer drugs.
Key Features:
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Safe: Non-radioactive assay.
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Sensitive and accurate: As low as 950 cells can be accurately quantified. Multi-well microplate reader.
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Convenient and high-throughput: “”Mix-incubatemeasure”” type assay. No wash and reagent transfer steps are involved. Safe: Non-radioactive assay.
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Sensitive and accurate: As low as 950 cells can be accurately quantified. Multi-well microplate reader.
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Convenient and high-throughput: “”Mix-incubatemeasure”” type assay. No wash and reagent transfer steps are involved.
Kit Components and Storage:
| Part |
Name |
TBS2003 |
| Unit Size |
| A |
MTT Assay Reagent |
10mL |
| B |
Solubilizer |
100 mL |
| Storage at –20°C |
| Shelf-life: 12 months after receipt. |
Related Products:
- Resazurin Cell Viability Kit (TBS2001)
- LDH Cytotoxicity Assay (TBS2002)
- MTS Cell Viability Assay (TBS2004)
- ATP Colorimetric/Fluorometric Assay Kit (TBS2010)
- ADP Colorimetric/Fluorometric Assay Kit
Research use only.
Documents
References
Gu, T., Lu, A., Wang, X., Brahan, N., Xu, L., Zhang, L., Su, K., Seow, K., Vu, J., Luk, C., Lee, Y., Raman, A., Pazzi, J., & Njoo, E. (2024, October 14). Synthesis and evaluation of Carmofur analogs as antiproliferative agents, inhibitors to the main protease (mpro) of SARS-COV-2, and membrane rupture-inducing agents. bioRxiv. https://doi.org/10.1101/2024.10.11.617727
Research use only.
