Monocyte Spin Medium, Density Gradient Medium

Monocyte Spin density gradient medium is a ready-to-use, sterile solution for the enrichment of monocytes from fresh sample material (whole blood, buffy coat). Depending on the starting material, purities of 60-80% monocytes can be achieved. Monocyte Spin is a cell-sparing alternative to bead-based purification systems for monocytes and is therefore particularly suitable for subsequent applications such as cultivation and differentiation.

Features

• Ready to use – The pluriSpin separation media are ready to use and can be used directly for cell separation.
• Replace – Alternative products such as Pancoll or Lymphoprep, for the enrichment of monocytes, can be substituted with Monocyte Spin.
• Compatible – The enriched cells can be used for downstream applications such as MACS, SepMate, RosetteSep, MojoSort.
• Combinable – The pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.

Separation Scheme

Distribution of blood cell populations as a function of density
Blood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.

Target cell population with monocyte spin density centrifugation
The density of the separation media defines the cut-off for the cells. Monocyte Spin Medium has a cut-off of 1.065 g/ml. All cells with a lower density, such as monocytes (2) and thrombocytes (1) , are enriched with Monocyte Spin Medium. All cells with a higher density, such as lymphocytes (3), basophil granulocytes (4), neutrophil granulocytes (5), eosinophil granulocytes (6) and erythrocytes (7) pass through the PLT Spin Medium and are depleted. First, the sample material is carefully layered onto the Leuko Spin density gradient medium. Mixing of the two phases must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The two layers of leukocytes are then transferred to a new tube and the cells are washed.

BEFORE
Preparation of monocytes with Monocyte Spin Medium. The sample material (1) is carefully placed on the monocyte spin medium (2).

AFTER
Layers after centrifugation. After centrifugation, the monocytes form a white layer (2) on the monocyte spin medium (3). Cells with a higher density, such as erythrocytes, granulocytes, lymphocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).

Specifications

Ordering Information