Mir-X™ miRNA First-Strand Synthesis Kit

This 20 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio's TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315.

Features

• First-strand cDNA synthesis from miRNA
• Quantitative PCR (qPCR) of miRNA

Applications

cDNA synthesis from microRNA
microRNA quantitation by qPCR

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• Components
• Components
• Components