Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA and RNA, especially at AT or AU rich regions, but will also digest double-stranded DNA or RNA. This enzyme digests 5'-phosphodiester bonds of DNA and RNA, and yields 3'-phosphate mononucleotides and oligonucleotides. It is commonly used for preparation of nucleosomes during epigenetic analysis studies. This enzyme requires Ca2+ and is completely inactivated by EDTA or EGTA.
Features
Digestion of nucleotides included in crude cell extract
Digestion of chromatin for nucleosome preparation
Chromatin immunoprecipitation (ChIP)
Applications
Digestion of nucleotides included in crude cell extract Digestion of chromatin for nucleosome preparation Chromatin immunoprecipitation (ChIP)
