Matchmaker® Gold Yeast Two-Hybrid System

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Features

• Identify novel protein interactions
• Confirm suspected interactions
• Define interacting domains

Applications

Yeast two-hybrid screening

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pGBKT7-Lam Control vector sequence in .gb format
• pGADT7 AD Vector Information
• pGADT7 AD Vector Sequence in GenBank Format
• pGBKT7 Vector Information
• pGBKT7 Vector Sequence in GenBank Format
• pGBKT7-53 Control vector sequence in .gb format
• pGADT7-T Control vector sequence in .gb format