Malondialdehyde (MDA) is a metabolite produced lipid peroxidation in living organisms. One molecule of MDA reacts with 2 molecules of 2-thiobarbituric acid (TBA) yield a 2-ThioBarbituric Acid Reactive Substances (TBARS), which is a chromophore with absorbance maximum at 532 nm.
TBARS is a known biomarker of oxidative status in a biological system, which involves many diseases, such as neurodegenerative diseases, cardiovascular diseases, and cancer.
The MDA Assay provides a sensitive, simple and accurate approach to determine MDA concentration in tissue, cells and other biosamples.
APPLICATION
Direct Assays: Quantitative detection of MDA in cell, tissue, plasma, serum, and urine samples.
KEY FEATURES
High-Throughput: Designed for 96-well plates, allowing multiple samples to be tested at the same time.
Simple and Rapid Procedure: The test process is straightforward and takes about 60 minutes to complete.
KIT CONTENTS
Name
Size
Lysis Buffer
25 ml
Assay Buffer
25ml
MDA Standard (1mM)
100 μL
Detector solution
25 ml
BHT stock (100x)
250 μL
STORAGE
Store kit at -20°C, avoid repeated freeze-thaw cycles.
