Mag-Bind® Endo-free Plasmid Mini Kit
Vendor: Omega Bio-tek
SKU: M1261-00
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Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.
- Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
- Versatile – Process up to 1.5 mL of different culture medias with different plasmid
- Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
| Component | Available Separately |
|---|---|
| Solution I | Solution I |
| Solution II | Solution II |
| N3 Buffer | |
| IRD Buffer | IRD Buffer |
| VHB Buffer | VHB Buffer |
| Endo-free Water | |
| Mag-Bind® Particles RQ | Mag-Bind® Particles RQ |
| RNase A | RNase A |
Components for optional protocol:
Specifications
| Starting Amount | up to 1.5 mL overnight bacterial culture per well of a 96-well deep well plate |
|---|---|
| Starting Material | Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium |
| Elution Volume | 50 - 100 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 1 - 96 |
| Processing Time | 96 samples in 95 minutes |
| Endotoxin levels | < 0.1 EU/µg |
| Purity | Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8 |
| Lysate Clearance Methodology | Magnetic beads or Centrifugation |
| Downstream Applications | Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA |
Documents
Specifications
- Binding technology Magnetic beads
- Downstream application Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA
- Elution volume 50 - 100 µL
- Processing mode Automated, Manual
- Processing time 96 samples in 95 minutes
- Starting amount up to 1.5 mL overnight bacterial culture per well of a 96-well deep well plate
- Starting material Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium
- Sub category Bacterial Plasmid
- Throughput 1 - 96
links and documants
- Protocol manual Click here
- Safety data sheet Click here
- Source url Click here
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Mag-Bind® Endo-free Plasmid Mini Kit
Regular price
0.00 NIS
Sale price
Regular price
Unit price
/per
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