Mag-Bind® Endo-free Plasmid Midi Kit
Vendor: Omega Bio-tek
SKU: M1272-00
Regular price
0.00 NIS
Sale price
Regular price
Unit price
/per
Quote Required
In stock
Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.
- Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding – Plasmid DNA yield up to 200 μg
- Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
- Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
- Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Included with each kit:
| Component | Available Separately |
|---|---|
| Solution I | Solution I |
| Solution II | Solution II |
| N3 Buffer | |
| IRD Buffer | IRD Buffer |
| VHB Buffer | VHB Buffer |
| Endo-free Water | |
| Mag-Bind® Particles RQ | Mag-Bind® Particles RQ |
| RNase A | RNase A |
Components for optional protocols:
Specifications
| Starting Amount | up to 50 mL bacterial culture in LB or up to 10 mL bacterial culture in TB |
|---|---|
| Starting Material | Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium |
| Elution Volume | 100 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 1 - 24 |
| Processing Time | 24 samples in ~ 70 minutes |
| Endotoxin levels | < 0.1 EU/µg |
| Purity | Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8 |
| Lysate Clearance Methodology | Magnetic beads, Centrifugation or Syringe |
| Downstream Applications | Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA |
Documents
Specifications
- Binding technology Magnetic beads
- Downstream application Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA
- Elution volume 100 µL
- Processing mode Automated, Manual
- Processing time 24 samples in ~ 70 minutes
- Starting amount up to 50 mL bacterial culture in LB or up to 10 mL bacterial culture in TB
- Starting material Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium
- Sub category Bacterial Plasmid
- Throughput 1 - 24
links and documants
- Protocol manual Click here
- Safety data sheet Click here
- Source url Click here
Couldn't load pickup availability
Mag-Bind® Endo-free Plasmid Midi Kit
Regular price
0.00 NIS
Sale price
Regular price
Unit price
/per
Quick order list
Free shipping on almost everything
Free Shipping
All orders over $500 are delivered to your doorstep at no extra charge.
30-Days Free Returns
Enjoy the freedom of stress-free shopping with our hassle-free and return policy.
Secure Payment
Shop with confidence knowing your payments are secure and your information