LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. The medium has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used serum-free media. T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies. LymphoONE medium also contains L-glutamine and streptomycin, and is confirmed to have low endotoxin levels (<0.3 EU/ml).
Features
Xeno-free medium that enables efficient T-cell expansion with or without the addition of serum or plasma
Supplemented with human serum albumin, recombinant human insulin, L-glutamine, and streptomycin
Endotoxin tested (endotoxin <0.3 EU/ml)
Compatible with CultiLife culture bags for large-scale expansion of T cells
Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin reagent* enhances proliferation and leads to a higher proportion of naïve T cells
Applications
Expansion of T cells Gene transfer by lentiviral or retroviral transduction Enhance culture and expansion of T cells and CAR-T cells
