Leuko Spin Medium, Density Gradient Medium

Leuko Spin density gradient medium is a sterile, ready-to-use solution for the isolation of human leukocytes from fresh sample material (whole blood or buffy coat) with high yield. Mononuclear and polymorphonuclear (polymorphonuclear) cells can be enriched with Leuko Spin Medium using a simple density gradient centrifugation. In combination with PBMC Spin Density Medium, themononuclear and polymorphonuclear cell fractions can be enriched simultaneously from one sample.

Features

• Ready to use – The pluriSpin separation media are ready to use and can be used directly for cell separation.
• Replace – Alternative products, such as GranuloSep, Pancoll or Histopaque - 1119, for the enrichment of leukocytes can be substituted with Leuko Spin.
• Compatible – The enriched cells can be used for downstream applications such as MACS, SepMate, RosetteSep, MojoSort.
• Combinable – The pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.

Separation Scheme

Distribution of blood cell populations as a function of density
Blood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.

Target cell population with Leuko Spin density centrifugation
The density of the separation media defines the cut-off for the cells. Leuko Spin Medium has a cut-off of 1.090 g/ml. All cells with a lower density, such as thrombocytes (1), monocytes (2), lymphocytes (3), basophil granulocytes (4), neutrophil granulocytes (5) are enriched with Leuko Spin Medium. All cells with a higher density, such as eosinophil granulocytes (6) and erythrocytes (7), pass through the Leuko Spin medium and are depleted. First, the sample material is carefully layered onto the Leuko Spin density medium. Mixing of the two phases (sample material and density medium) must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The two layers of leukocytes are then transferred to a new tube and the cells are washed.

BEFORE
Preparation of leukocytes with Leuko Spin Medium. The sample material (1) is carefully placed on the Leuko Spin Medium (2).

AFTER
Layers after centrifugation. After centrifugation, the leukocytes form two white layers on the Leuko Spin Medium (4). Layer (2) consists of mononuclear leukocytes and layer (3) of polymorphonuclear leukocytes (granulocytes). Cells with a higher density, such as erythrocytes, and dead cells pass through the medium and are located at the bottom of the tube (4).

Specifications

Ordering Information