Lenti-X™ 293T Cell Line

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with Lenti-X Packaging Single Shots and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108 ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen.

Features

• High-titer lentiviral vector system
• Constitutive expression from the human EF1 alpha promoter
• Robust, long-term expression, in the broadest range of cell types
• Bright fluorescent proteins to monitor expression or localization, learn more from our fluorescent protein quick guide.

Applications

If CMV promoters are silenced in your cell type, try EF-1 alpha
Lentiviral transduction of stem cells
Lentiviral transduction of hematopoietic cells

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pLVX-EF1a-IRES-ZsGreen1 vector sequence in GenBank format