LDH Cytotoxicity Colorimetric Assay

Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme which is present in most cells. It is released upon damage to the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate cell membrane integrity and quantify cell cytotoxicity.

The Tribo™ LDH Cytotoxicity Colorimetric Assay Kit uses formazan from oxidized WST-8 to measure the activity of LDH released from damaged cells. The generated signal at OD 460 nm is proportional to the number of lysed cells. The kit provides the easiest and most accurate approach to measure LDH activity and cytotoxicity of a variety of compounds.

Applications

• Cell Proliferation.
• Cytotoxicity and LDH activity in cells and tissue.

 

Storage Conditions

Store the Reagent at -20°C protected from light. Shelf life: 6 months.

 

Fig. 1: LDH Assay Principle

 

Fig. 2: LDH Standard Curve

 

Related Products

1. Resazurin Cell Viability Kit (TBS2001)
2. CCK-8 Cell Viability Assay (TBS2022)
3. ATP Colorimetric/Fluorometric Assay Kit (TBS2010)
4. ADP/ATP Ratio Assay Kit (Bioluminescent (TBS2015)
5. ADP Colorimetric/Fluorometric Assay Kit 

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)