Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK). Cat. # 2140B contains 5 of Cat. # 2140A. Please refer to Cat. # 2140A for complete product documentation and resources.
Features
Random primer labeling
Second-strand cDNA synthesis
Dideoxy DNA sequencing
2140AK/BK are sequencing grade reagents
Applications
Random primer labeling Second-strand cDNA synthesis Dideoxy DNA sequencing 2140AK/BK are sequencing grade reagents
